Selection of inserted transgenes works by the following two steps An incorporate

Question

Selection of inserted transgenes works by the following two steps

An incorporated neomycin resistance cassette provides resistance to G-418.

Cells expressing tkHSV which is not incorporated into genomic DNA die due to cytotoxicity

of ganciclovir

An incorporated neomycin resistance cassette causes cells to die because of ganciclovir.

A and B

An incorporated neomycin resistance cassette provides resistance to G-418.

Cells expressing tkHSV which is not incorporated into genomic DNA die due to cytotoxicity

of ganciclovir

An incorporated neomycin resistance cassette causes cells to die because of ganciclovir.

A and B

Explanation / Answer

Option 4. A and B

G418 (Geneticin) is an aminoglycoside antibiotic. Resistance to G418 is conferred by the neo gene from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APT 3' II. Ganciclovir is a kind of negative selection that eliniminates the growth of cells upon selection.

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