To perform inside out PCR the following steps are required Remove the known targ

Question

To perform inside out PCR the following steps are required

Remove the known target gene using restriction enzymes, ligate the cut DNA,

perform PCR using primers to the known sequence

Remove the known target gene using restriction enzymes and perform PCR using a reverse

transcriptase

Remove the known target gene using restriction enzymes and use random primers to

amplify the unknown gene

Remove the known target gene using restriction enzymes, ligate the cut DNA,

perform PCR using primers to the known sequence

Remove the known target gene using restriction enzymes and perform PCR using a reverse

transcriptase

Remove the known target gene using restriction enzymes and use random primers to

amplify the unknown gene

Explanation / Answer

Remove the known target gene using restriction enzymes, ligate the cut DNA, perform PCR using primers to the known sequence.....

Also known as Inverse PCR, in inside out PCR, the target sequence is first cut using restriction endonuclease, then this sequence is allowed to ligate, forming a circular DNA. Primer of known region of DNA is constructed and the task can be performed by using a single primer.. No forward reverse primer required.

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