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write the complementary sequence for the DNA template below and indicate the 5\'

ID: 612435 • Letter: W

Question

write the complementary sequence for the DNA template below and indicate the 5' end and 3' of the complementary strand. 5' A-C-C-G-C-G-A-C-C-C-A-A-A-G-G-A-T-T-T-G-G-T-A-T-T-G-C-C-C-G-G-C-A-T-A 3'

Explanation / Answer

. The DNA strand below carries the code for an amino acid sequence. Write out the complementary DNA strand to the strand below. Be sure to label the 5’ and 3’ ends. Identify the triplet on the DNA that corresponds to the start codon on the transcript that will be made from this DNA. 5’ ….TCATGGACCAATAGG….3’ > The complementary strand of DNA will be 3'....AGTACCTGGTTATCC....5' The triplet corresponding to the start codon is TAC B. Write out the mRNA sequence that can be produced from this DNA that would carry a start codon for translation and additional amino acids. Which strand of your doublestranded DNA is the template for transcription (the one the RNA polymerase moves along and reads)? Mark the start codon of your transcript. > The template strand for the mRNA to be produced will be of polarity 3' to 5'. 3'....AGTACCTGGTTATCC....5' mRNA produced from this will be 5'....UCAUGGACCAAUAGG....3' The UC will be a UTR (Untranslated region) and will be left for an efficient transcription. The start codon of transcript is AUG. C. The start codon on mRNA is AUG but the anticodon present on the tRNA is UAC. Write the codon and anticodon below and label the 5’ end and the 3’ end of each. On mRNA codon is 5'... AUG...3' On tRNA anticodon will be 3'....UAC....5'