Need an explanation, not just an answer. Confocal fluorescence microscopy differ

Question

Need an explanation, not just an answer.

Confocal fluorescence microscopy differs from epifluorescent microscopy in what way?

The specimen in confocal microscopy must be fixed

The detector in confocal microscopy does not detect fluorescence

The source in confocal microscopy simultaneously impinges upon multiple focal planes

The source in confocal microscopy impinges upon a single focal plane

The specimen in confocal microscopy must be fixed

The detector in confocal microscopy does not detect fluorescence

The source in confocal microscopy simultaneously impinges upon multiple focal planes

The source in confocal microscopy impinges upon a single focal plane

Explanation / Answer

c. The source in confocal microscopy simultaneously impinges upon multiple focal planes. ----------> TRUE

a. The specimen in confocal microscopy must be fixed -----------------> Confocal microscopy can also be used to study live cells, but not only fixed specimens.

b. The detector in confocal microscopy does not detect fluorescence --------> The detector in confocal fluorescence microscopy can detect fluorescence.

d.  The source in confocal microscopy impinges upon a single focal plane ---------------------> The source in confocal microscopy impinges upon multiple focal planes.

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