........... Picture below shows the banding patterns obtained by sequencing a pa

Question

........... Picture below shows the banding patterns obtained by sequencing a part of the gene responsible for Cystic Fibrosis from a healthy person and the same part of the gene from a person with Cystic Fibrosis. (Sequencing was carried out by setting up of 4 separate dideoxy reactions for each of the two DNA samples and then, the products were separated by gel electrophoresis) From the picture, determine the sequence of the normal copy of the gene and the sequence of the mutated gene. Normal gene sequence: Mutated gene sequence: If this particular mutation is a 3bp deletion, identify with an arrow on the above mutated sequence the location of the mutation that causes cystic fibrosis. Which three bases were deleted?

Explanation / Answer

a)

Normal gene sequence: ATAGTAGAAACCACAAAGGATACTA

Mutated gene sequence: ATAGTAACCACAAAGGATACTACTT

b)

Normal gene sequence: ATAGTAGAAACCACAAAGGATACTA

Mutated gene sequence: ATAGT--AACCACAAAGGATACTACTT

c)

The bases AGA were removed from normal gene sequence and new bases CTT were added in the mutated gene sequence.

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