You have developed a novel antibiotic, which you intend to call Hulkomycin in ho

Question

You have developed a novel antibiotic, which you intend to call Hulkomycin in honor of your favorite superhero. You decide to study its mechanism of inhibition using a poly-ribonucleotide with the following sequence:

5’ – AUG UUU UUU UUU UUU UUU UUU UUU UUU – 3’

You add this RNA to a cell-free extract that has all of the components of the cell without the cell-wall. When you don’t add Hulkomycin, you can obtain a nonapeptide (a peptide with 9 amino acids). In the presence of your antibiotic, you only see a dipeptide – fMet-Phe. Answer each of the following questions based on your understanding of translation.

What step in polypeptide synthesis is inhibited by Hulkomycin?

Which ribosomal site do you expect the nonapeptide to be in at the end of the uninhibited reaction?

Do you expect to find the dipeptide attached to a tRNA at the end of the inhibited reaction?

Do you think that Hulkomycin will interact with the N-terminus or the C-terminus of the resulting protein?

Explanation / Answer

In presence oof hulkomycin only dipeptide is synthesized it means that this antibiotic some how disturb the process of translocation during poly peptide synthesis. since hulkomycin does not affect the process of first peptide bond formation but for further peptide bond formation there must be process i.e. translocation occuered so that p- site of ribosome could vacant for incoming charged polypeptide.

so it has greater probeblility that this antibiotic terminate the translation process after dipeptide synthesis and released dipeptide containing t- RNA from ribosome.

we cannot predict hulkomycin association N- terminal or C- terminal by given information.

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