1. The genotypes of four strains of Neurospora are unknown. However, we do know

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1. The genotypes of four strains of Neurospora are unknown. However, we do know that all strains require arginine and have the growth characteistics shown below. Nutrition and Accumulation Growth on inimal Omithine lin ine Accumulate Ornithine Citrulline Citrulline Ornithine Pairwise complementation tests of the four strains: += heterokaryon grows on minimal media 0= no growth of heterokaryon on minimal media 3 0 0 4 + Crosses among mutants yielded pro to trophs in the following percentages: 1 x 2 25% 1 x 4 none detected among 1 million ascospores 1x3 2596 2x4 0.00196 2 x 3 0.002% 3 x 4 none detected among 1 million ascospores A. How many distinct mutational sites are represented among these four B. Give the genotypes of the four strains, using a consistent and C. Give the map distances between all pairs of linked mutations. strains affecting how many polypeptide chains? infor mative set of sy mbols. D. What percentage of prototrophs would be expected among ascospores of the following types: Strain 1x wild type Strain 2 x wild type Strain 3 x wild type Strain 4 x wild type

Explanation / Answer

% of prototrophs expected among ascospore between strain 1 and wild type is

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