QUANTITATIVE Imagine that you are repeating the replica-plating procedure of Jac

Question

QUANTITATIVE Imagine that you are repeating the replica-plating procedure of Jacob and Monod to find mutants that can't grow using lactose. After treating cells with a mutagen, you anticipate a mutation rate of 1×105 lactose-nonutilizing mutants per mutagen-treated cell.

Part A

Imagine that you are repeating the replica-plating procedure of Jacob and Monod to find mutants that can't grow using lactose. After treating cells with a mutagen, you anticipate a mutation rate of 1×105 lactose-nonutilizing mutants per mutagen-treated cell.

Based on this estimate, how many cells should you replica-plate to have a good chance of finding at least one mutant?

Explanation / Answer

At the mutation rate of 1 x 10^-5 per cell, the average no. of mutants would be 10,000 (1 x 10^5) cells. So, there is possibility to find one mutant in possibility of 10,000 cells

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