1. One of the most common method for detecting a protein of interest relies on a

Question

1. One of the most common method for detecting a protein of interest relies on a __________________ probe and is called a ____________________.

Select one:

a. antibody; western blot

b. single stranded RNA; northern blot

c. none of these are correct

d. single stranded DNA; southern blot

2. A cDNA library is produced from ______________ and is valued in the analysis of __________________.

Select one:

a. a DNA copy of mRNA isolated from a group of cells; the protein-encoding open reading frame of a gene (no introns)

b. chromosomal DNA digested with restriction enzymes; the promoter and regulatory elements of a particular gene

c. a DNA copy of mRNA isolated from a group of cells; the promoter and regulatory elements of a particular gene

d. protein sequences isolated from particular tissues; enzyme function encoded by a particular gene

e. chromosomal DNA digested with restriction enzymes; chromosomal organization

3. Huntington's disease is one class of genetic diseases called 'triplet repeat disorders'. These disorders occur when a repeat of three nucleotides gets amplified by errors during DNA replication. People with a small number of repeats (10-20) don't get the disease while people with larger numbers of repeats (80-200) do get the disease. You want to design PCR primers to help directly determine the number of repeats that a person has. The region in which the repeat occurs is shown below (the repeat region is in bold).

5' CGTGTCGTAAGCTCCCAGCAGCAGCAGCAGAACTCCAGCGGTAGGC 3'

3' GCACAGCATTCGAGGGTCGTCGTCGTCGTCTTGAGGTCGCCACCGT 5'

The best primers to amplify this region would be

a. 5'CGTGTCGTAAGCTCC3' and 5'AACTCCAGCGGTAGGC3'

b. 5'CGTGTCGTAAGCTCC3' and 3'AACTCCAGCGGTAGGC5'

c. 3'GCACAGCATTCGAGG5' and 5'AACTCCAGCGGTAGGC3'

d. 5'CGTGTCGTAAGCTCC3' and 5'GCACAGCATTCGAGG3'

e. 5'CGTGTCGTAAGCTCC3' and 5'TGCCACCGCTGGAGTT3'

Explanation / Answer

1. a. antibody; western blot.

2. a. a DNA copy of mRNA isolated from a group of cells; the protein-encoding open reading frame of a gene (no introns)

3. The best primer to amplify this region would be

e. 5'CGTGTCGTAAGCTCC3' and 5'TGCCACCGCTGGAGTT3'

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