1.Which of the following probes will hybridize to this target sequence of DNA? 5

Question

1.Which of the following probes will hybridize to this target sequence of DNA?

5' - AGG TGA CCG TTA ACG CCT - 3'

5' - AGG CGT TAA CGG TCA CCT - 3'

5' - TCC ACG GGC AAT TGC GGA - 3'

5' - TCC GCA AAT GCC AGT GGA - 3'

5' - UCC UCG CCG TTA ACG CCT -

2.

Messenger RNA (mRNA) has a sequence (5' to 3') which exactly corresponds (with the substitution of U for T) to the sequence of the:

ribosomal RNA (rRNA)

template strand of DNA

transfer RNA (tRNA)

coding strand of DNA

3.

What process can be used to make a DNA probe produce the fluorescence of chemiluminescent signal?

Enzymatic attachment of acridinium esters to terminal ends of the probe

Splicing the gene for beta-lactosidase into the probe

Substitution of biotinylated or fluorescent nucleotides into the probe

Heat denaturation of the probe followed by acid treatment

4.

What term describes the products produced when DNA is digested by restriction endonucleases?

Chimeras

Amplicons

Restriction fragment length polymorphisms

Mossaicisms

5.

Which statement accurately describes the process of fluorescent in situ hybridization (FISH)?

Hybridization is performed directly on intact chromosomes.

Hybridization occurs by attachment to the probe only at the centromere.

Hybridization probes are attached to histones associated with the chromosomes.

Hybridization is performed on DNA extracted from cells.

6.

Which of the following is the most common type of polymorphism?

Singular nucleotide polymorphism (SNP)

Short tandem repeat (STR)

Short repetitive interspersed element (SINES)

Variable number tandem repeat (VNTR)

5' - AGG CGT TAA CGG TCA CCT - 3'

5' - TCC ACG GGC AAT TGC GGA - 3'

5' - TCC GCA AAT GCC AGT GGA - 3'

5' - UCC UCG CCG TTA ACG CCT -

Explanation / Answer

1) b is the answer, 5' - TCC ACG GGC AAT TGC GGA - 3' . There is single nucleotide mismatch in the probe but then also probe will hybridize with the sequence 5' - AGG TGA CCG TTA ACG CCT - 3'

2) d is the answer, coding strand of DNA because the coding strand is exactly the same as m rna with differences in adenine being replaced with uracil.

3) a is the answer, (Enzymatic attachment of acridinium esters to terminal ends of the probe) because chemicals are attached to the probe which fluoresces after enzymatic reaction.

4) c is the answer,(restriction fragment length polymorphisms) because it creates fragments of DNA.

5) a is the answer, (Hybridization is performed directly on intact chromosomes.)

6) a is the answer, (Singular nucleotide polymorphism,SNP)

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