Multiple choice questions about a graph of limiting substrates Consider the grow
ID: 60195 • Letter: M
Question
Multiple choice questions about a graph of limiting substrates
Consider the growth of Pseudomonas sp. in a chemostat operating at steady state. Each of two potentially growth-limiting substrates, glucose and acetate, are present in the (sterile) nutrient feed to the bioreactor, at S_0, g/u = 400 mg/L and S_0,acet= 2400 mg/L. The respective mu vs. S behavior in each case is plotted below. While the two patterns are quite dissimilar, they happen to intersect at S = 95 mg/L. Monod kinetics are followed during acetate-limited cell growth, while glucose-limited growth is accompanied by glucose inhibition. Product formation is entirely growth-associated, and cell maintenance is achieved only through endogenous metabolism. Suppose the steady state substrate concentrations in the chemostat are S_glu = 20 mg/L and S_acet, = 5.0 mg/L. Identify which of the following is TRUE: We now increase flow rate and reach a new steady state where S_acet, = 95 mg/L. Identify which of the following is TRUE: We increase flow rate again, and reach a new steady state where S_acet, = 200 mg/L. Identify which of the following is TRUE: We increase flow rate yet again, and reach a new steady state that is just prior to the washout condition, where S_acet is very near 2400 mg/L. Identify which of the following is TRUE:Explanation / Answer
4a. (iii) glucose and acetate are both limiting
b. (ii) glucose is limiting
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