1. The polymerase chain reaction requires single stranded DNA primers that: a. a

ID: 60128 • Letter: 1

Question

1. The polymerase chain reaction requires single stranded DNA primers that:

a. anneal to each other to prime DNA polymerization.

b. hybridize only to DNA within open reading frames.

c. anneal to the same strand of template DNA, though at distant sites.

d. anneal to opposite strands of template DNA at distant sites, with their 5’ ends directed toward each other.

e. anneal to opposite strands of template DNA at distant sites, with their 3’ ends directed toward each other.

2. In the Sanger sequencing method, the use of dideoxy adenosine triphosphate stops nucleotide polymerization:

a. opposite G’s in the template strand.

b. opposite T’s in the template strand.

c. opposite A’s in the template strand.

d. opposite any base selected randomly in the template strand.

e. opposite C’s in the template strand.

3. When using reverse transcriptase, DNA is made from mRNA.

Select one:

True

False

4. The intermediate temperature cycle (72oC) in the polymerase chain reaction enables activity of the thermostable DNA polymerase enzyme.

Select one:

True

False

Answer 1- e (anneal to opposite strands of template DNA at distant sites, with their 3’ ends directed toward each other).

Answer 2- b ( opposite T’s in the template strand.)

Anser 3- True

Ans 4- True

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