The problem below relates to the synthesis of several intermediates in the citri
ID: 59807 • Letter: T
Question
The problem below relates to the synthesis of several intermediates in the citric acid cycle, which is essential in the production of ATP through aerobic respiration. A set of experimental results relating the growth (+) of Neurospora on several media is given in the table. Based on the information provided, present the biochemical pathway for the substances oxaloacetate, fumarate, malate, and succinate, and the locations of the metabolic blocks produced by the various strains. Strain MM MM +oxaloacetate MM +fumarate MM +malate MM +succinate 162 - + - + - 136 - + - - - 141 - + + + - MM = minimal medium What is the order of mutation?
Explanation / Answer
In the Krebs cycle the order of the formation of different substances
Succinate----> Fumarate----> Malate----> Oxaloacetate.
Strain 162 grows well in oxaloacetate and malate is supplemented. So the mutation is in the enzyme converting fumarate to malate.
Strain 136 grows only if oxaloacetate is supplemented. The mutation is in the enzyme converting malate to oxaloacetate
Strain 141 grows well only if it is supplemented with all substances. The mutation is in the enzyme forming the succinate.
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