1. The Shine-Dalgarno site is present in bacterial mRNA to a. direct the ribosom

Question

1. The Shine-Dalgarno site is present in bacterial mRNA to

a. direct the ribosome to the proper position on the mRNA for translation initiation

b. help the ribosome determine which tRNA to incorporate

c. assist the elongation factors

d. form the A site in the ribosome

e. tell the translation machinery when to stop translation

2. Which statement best describes the general process of regulation by gene activiation?

a. the activator binds to the operator and blocks RNA polymerase under all conditions

b. the activator binds to the operator and blocks RNA polymerase under some conditions

c. the activator binds to the repressor and cause it to fall off the DNA

d.the activator binds to the promoter and initiates transcription

e. the activator binds near the promoter and facilitates transcription initiation

3. You are studying transcriptional regulation in the arginine biosynthesis operon. You know that it is regulated by a repressor protein, ArgR, which binds the corepressor molecule, arginine. When arginine is present in excess growth needs you would expect that

a. arginine is not bound to the repressor protein

b. arginine would bind to the operator sequence

c. ArgR would bind arginine and thus be able to bind the operator site

d. ArgR would be degraded by the cell to allow for more arginine synthesis

e. there would be increased expression of the arginine biosynthesis operon

4. In a gene regulated by a positive activator, what is the typical situation when the inducer molecule is present?

a. RNA polymerase transcribes the gene at a high level

b. there is very little transcription of the gene

c. the positive activator is not able to bind to the inducer

e. none of these are likely to happen

Explanation / Answer

1)a

2)e

3)c

4)a

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