1. You plan to perform artificial DNA synthesis using oligonucleotides for const
ID: 59608 • Letter: 1
Question
1. You plan to perform artificial DNA synthesis using oligonucleotides for construction of a synthetic gene. The sequence of the desired gene is shown below. The synthesis reaction can be performed at three different scales providing a total of 40 nmole, 1 µmole, or 10 µmole of starting material (i.e., synthesis at 100% yield would generate 0.04, 1, or 10 µmol of product, respectively). The cost of synthesis differs for each scale: $0.50/base, $1.00/base, and $2.00/base, respectively. The coupling efficiency of each nucleotide addition step is 98.3%. To assemble your oligonucleotides into the full-length gene, you require at least 28 nmol of the correct oligonucleotide product and you will need at least 15 bases of overlap between oligonucleotides for hybridization. What is the most economical synthesis scale (of the three possibilities) at which the oligonucleotide synthesis reactions can be conducted? Draw boxes on the sequence below to indicate the sequences of the oligonucleotides you would use
Explanation / Answer
The most economical synthesis scale at which the oligonucleotide synthesis reactions can be conducted is $0.50/base. This is because we can get 100% yield with 98.3% coupling efficiency and the product has 0.04 µmol. Where, we are getting 100% product with low cost, when compared to other two scales. However, we need only 28 nmol of product, So, 40 nmole scale is the best way.
This is because the adenine and thymine use less energy for bonding.
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