1. The DNA sequence below represents a gene in double-stranded eukaryotic DNA. T
ID: 59458 • Letter: 1
Question
1. The DNA sequence below represents a gene in double-stranded eukaryotic DNA. There is a partial promoter, a transcription start site, a series of codons and a transcription termination site (polyadenylation site).
Assume that the -25 position is at the very last nucleotide of the TATA box.
5’ AAGCTCGATTTATT CCATGG ACTAGTTCAGCATCGTAAGGCACGGAGGCTCGATTCGGATCGATTT TATAGCGAAT 3’
3’ TTCGAGCTAAATAAGGTACCT GATCAAGTCGTAGCAT TCCGTGCCTCCGAGCTAAGCCTAGCTAAAATATCGCTTA 5’
a. Write out the mRNA sequence transcribed from this gene (give the polarity of the molecule). You will not be penalized if your transcription start site varies from the answer by one or two nucleotides. Continue transcription until 5 nucleotides past the end of the polyA signal. 2 pts
b. Write out the amino acid sequence (give the polarity of this molecule too) encoded within this mRNA. 2 pts
Explanation / Answer
1. The DNA sequence below represents a gene in double-stranded eukaryotic DNA. T
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