22. How much of 10x TAE buffer do we need to make 500 mL of solution? (4 pts) 23

Question

22. How much of 10x TAE buffer do we need to make 500 mL of solution? (4 pts) 23. We have 10 L of 3 M CaCl and want to make 25 mM CaCl solution. How much DI H.0 must we add? (4 pts) 24. We have 25 L of a 200 ppb Potassium Phosphate (K-HPO) solution. If we used 20 mL of stock solution to make this solution, what is the concentration (in parts notation: 4 pts, X notation: 4 pts, and molarity: 4 pts) of the initial stock? (12 pts total) 25. Now much 6x loading buffer do you add to 10 L of DNA? (4 pts) 26. What is the concentration of a solution made by taking 50 g of NaCl and QS to 100 mL? ve the answer in molari

Explanation / Answer

22. Preparation of the 10X TAE Buffer:

1. Dissolve the Tris(hydroxymethyl)aminomethane, glacial acetic acid and EDTA in 800 mL of deionized water (DI water).

2. Dilute the buffer to 1 L (1000 mL). You need not sterilize the solution.

Therefore, for making 500 mL of solution, you need to use 400 mL of 10X TAE buffer.

23. Formula: M1V1 (for concentrated solution) = M2V2 (for dilute solution)

i.e. 3 M * 10 L = 25*10-3 M * V2

i.e. V2 = 1200 L

Therefore, the volume of DI water that must be added = V2-V1 = 1200 - 10 = 1190 L

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