7. Consider the following two improving the separation. In (a) the original chro

Question

7. Consider the following two improving the separation. In (a) the original chromatogram shows overlapping peaks. In (b) there is an increase in band separation, and in (c) there is a decrease in elution peak width. -component chromatograms, which illustrates two meth ods of 12 Time a) What experimental/instrumental modifications would increase band separation (resolution) from chromatogram (a) to chromatogram (b)? b) What experimental/instrumental modifications would decrease band width from chromatogram (a) to chromatogram (c)?

Explanation / Answer

Chromatographic separation,

a) transition from (a) to (b) chromatogram, the resolution can be increased by reducing the sample flow rate. This would give sufficient time for the samples to separate and give better resolution,

b) transition from (a) to (c) chromatogram can be achieved by reducing the sample concentration and the sample flow rate. Both of these would increase the resolution and band width of the peaks on the column.

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