You have been given the following results from a chromatogram on several related

Question

You have been given the following results from a chromatogram on several related strains: If you are told that the spots represent compounds in the same biosynthetic pathway then: What compound (1-5) is likely to be the final product? Which is likely the initial precursor? Which strain (A-E) is likely to be the wild type? What is the likely order of the five compounds in the biosynthetic pathway. If we assume that each of the mutant strains have lost one enzyme in the biosynthetic pathway show which mutants are missing which enzyme.

Explanation / Answer

Q1 what compound (1-5) is likely to be the final product?

ANS.The final product is 4

Q2 which is likely the initial precursor

ANS.Compound 3 is the initial precursor

Q3. which strain (A-E) is likely to be the wild type

ANS.Strain A is the wild type

Q4.what is the order of the five compounds in the biosynthetic pathway?

ANS. 3 ==> 5 ==> 1 ==> 2 ==> 4

Q5. If we assume that each of the mutant strains have lost one enzyme in a biosynthetic pathway, show which mutants are missing which enzyme.

ANS. 3 ==> 5 ==> 1 ==> 2 ==> 4
             B D C E

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