You are interested in how expression of YFGI is regulated In several different c

Question

You are interested in how expression of YFGI is regulated In several different cell types. you have discovered that there are three different regions of the YFGI promoter (A,B, and C) that are important in regulating gene expression in different cell types. below is a schematic of the gene for YFGI and it's upstream regulatory regions. (In picture)
-You decided to investigate the role of these three regulatory regions by cloning the DNA sequence upstream of YFGI and fusing it to a reporter gene. Please answer A, B, C, and D in the pictures

L. You are interested im hom eprson of Yhul is regulatod in several diterent cell types. have discovered that thero are three different regions of the YFG1 pro moter (A-Band C) diag important in regulating gene expression in different oell types Below is a schematic of thr e for YFG1 and its upstream regulstory regions You decide to investigate the role of these theee regulatory regicns by cloning the DNA sequence upstream of YFG1 and fusing it to a reponter gene. A) What reponser gene would you use if you wieted to read out expression using fruceescent After making the reparter construct you decide to mutate the difTerent regulatory regions in ander o desemine their relative contribotions to the expression of YFG1 in the cell types indicated below. Your data is summarized in the table below B) Does sequence A aetivate expression of YFGI or repress expression of YFG1? (2 points)

Explanation / Answer

A) Green fluorscent protein (GFP) can be used as reporter gene.

B) Sequnece A is activated the expression of YFG1, but is its activity is tissue specific.

C) protein Z is repressor and it is expected to be present in Skin tissue.

D) Protein Y is an activator and is expected to be present in Muscles.

Related Questions

Navigate

• Browse (All)
• Browse Y
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.