A temperate phage has the following sort of control circuit for making the decis

Question

A temperate phage has the following sort of control circuit for making the decision between repression or lytic growth after infection: Both P and p2 are equally accessible to the host RNA polymerase, so the lysogeny vs. lysis decision is random, depending on whether repressor1 or repressor 2 is made first (leading to lysogeny or lysis, respectively). (a) For each growth pattern shown below, determine which mutation(s) in the accompanying list would give that pattern. owth Pattern (30 Su(42) u (lysogenic) wild type type A type B type C type D type E tu turbid plaques cl clear plaques 0 = no plaques tu c1 cl cl cl 1. P1(enhanced initiation at p) 6. P2 (enhanced initiation at p2) 2. 0 (insensitive to repressor 2) 7. 2 insensitive to repressor 1) 3. R1ts (temperature-sensitive 8. R2 ts (temperature-sensitive 9. R2a (nonsense mutation in 10. ts mutation in an essential lytic repressor 1) repressor 2) R1 -am (nonsense mutation in repressor 1) repressor) 5. P2 (no initiation from P2) gene

Explanation / Answer

Wild-type: No initiation from P1

Type A: Temperature sensitive repressor 2 & repressor 1

Type B: Temperature sensitive repressor 1, No initiation from P2

Type C: Nonsense mutation of repressor 1, enhanced initiation at P2

Type D: Insensitive to repressor 2, insensitive to repressor 1

Type E: Mutation in an essential lytic gene,

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