3:59 PM twu.blackboard.com .\'I Sprint 58% Page 70 of 72 71 of 72 Name SectionDa

Question

3:59 PM twu.blackboard.com .'I Sprint 58% Page 70 of 72 71 of 72 Name SectionDae Lab Partners Data Sheet Comparing with the peotein marker, how large is the polypeptide in cach staining spott You mary count only the obvious bands Postlab Questions 1 Determine the molecular weight of LDAH. Does it concoed with its theoretical sine? 2What can the band intensity sell you? 3 How can you know whether there is any disulfide bond fomed in your protcin Design an exper- imem so verify your idea Grade TA: Page 71 of 72 References Experiments in Biochemistry: A Hands-On-Appreach by Farrell and Tarylor; 2 ed 2006 Lekwinger Principles of Biochemistry by Nelson and Cox;6 ed 2013 Molecmlar Cloningz A Laboratory Manwal by Green and Sambrook: 4 ed 2012.

Explanation / Answer

1. Lactic Dehydrogenase (LDH) has a total molecular weight of 140 kDa

Yes, they are related to their theoretical size.

Basically, It is composed of 4 subunits

They are designated M subunit (muscle) and H subunit (heart). These subunits may be mixed in any of 5 combinations (M4, M3H1, M2H2, MH3, and H4).

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