- Translation is an energy intensive process. What is the primary molecule used
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Question
- Translation is an energy intensive process. What is the primary molecule used for energy in translation? To translate a 100 amino acid protein, about how many of these molecules would be needed? How does energy utilization ensure proper order and progression of events?
- There is a critical step in initiation that alters the shape of the mRNA molecule to help a terminating ribosome rebind and translate the same mRNA again. What is the critical step? What fundamental principle of chemistry does it use to enhance efficienty?
Explanation / Answer
Ans -1. Mainly ATP and GTP are used. For an amino acid 1 ATP is used in activation , 2GTP are used in initiation and elongation . So total 3 molecules are used . For 100 amino acid 100 ATP and 200 GTP are used. And for termination 1 GTP is used. Ans-2 releasing factor recognizes the stop codon and binds to it along with GTP which hydrolysis the GTP into GDP and Pi and causes ribosomal peptidyl transferase to catalyze the nucleophillic attack by a molecule of water.
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