The DNA sequence below represents a gene in double-stranded eukaryotic DNA. Ther

Question

The DNA sequence below represents a gene in double-stranded eukaryotic DNA. There is a partial promoter, a transcription start site, a series of codons and a transcription termination site (polyadenylation site).

Assume that the -25 position is at the very last nucleotide of the TATA box.

5’AAGCTCGATTTATTCCATGGACTAGTTCAGCATCGTAAGGCACGGAGGCTCGATTCGGATCGATTTTATAGCGAAT 3’

3’TTCGAGCTAAATAAGGTACCTGATCAAGTCGTAGCATTCCGTGCCTCCGAGCTAAGCCTAGCTAAAATATCGCTTA 5’

a. Write out the mRNA sequence transcribed from this gene (give the polarity of the molecule). You will not be penalized if your transcription start site varies from the answer by one or two nucleotides. Continue transcription until 5 nucleotides past the end of the polyA signal.

Explanation / Answer

* Based on the presence of TATA box, the template strand and the mRNA transcribed is as follows:

The template strand has the sequence:

3' TAAGCGAGATATTTTAGCTAGGCTTAGCTCGGAGGCACGGAATGCTACGACTTCATCAGGTACCTTATTTAGCTCGAA 5'

The mRNA strand has the following sequence:

5' AUUCGCUCUAUAAAAUCGAUCCGAAUCGAGCCUCCGUGCCUUACGAUGCUGAAGUAGUCCAUGGAAUAAAUCGAGCUU 3'

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