The kinetic data given below are for an enzyme in the absence and presence of a

Question

The kinetic data given below are for an enzyme in the absence and presence of a reversible inhibitor. From the data, generate both a Michaelis-Menten and Linweaver-Burk Plot for both that uninbibited and inhibited reactions. Graph both the uninhibited and inhibited data on the same plot. From these data calculate the Vmax and Km for the enzyme in absence and presence of the inhibitor. Is the inhibitor working cometitively or noncompetitively? Explain.

[S], mM Vo, mM/min   Vo, mM, min with inhibitor

0.5           23.5               16.67

1.0           32.2                25.25

1.5           36.9               30.49

2.5           41.8                37.07

3.5           44.0                38.91

Explanation / Answer

The Vmax remained constant but there is an increase in Km when the inhibitor is present. This indicates a competetive inhibition

[S], mM 1/S Vo, mM/min 1/Vo Vo, mM, min with inhibitor 1/Vo inh+ 0.5    2.0 23.5 0.043 16.67 0.06 1 1.0 32.2 0.031 25.25 0.04 1.5 0.7 36.9 0.027 30.49 0.03 2.5 0.4 41.8 0.024 37.07 0.03 3.5 0.3 44 0.023 38.91 0.03

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