Determine if the following statements are true or false. 1. The differences in t

Question

Determine if the following statements are true or false.

1. The differences in transcription between prokaryotes and eukaryotes occur mainly in the initiation and termination steps, while the elongation step is fairly similar between the two.
2. Eukaryotes use a single RNA polymerase to transcribe different categories of genes while prokaryotes have three.
3. Eukaryotic transcriptional termination may occur via the rho-dependent model or rho-independent model.
4. Eukaryotic initiation of transcription occurs when the five general transcription factors and RNA polymerase assemble together to form an open complex.
5. Eukaryotic promoters have a core promoter and regulatory elements such as enhancers and silencers.

Explanation / Answer

1. True, The initiation in eukaryotes requires the formation of an initiation complex with different transcription factors but prokaryotes do not require any such driving factors for initiation. The termination in prokaryotes is either rho-dependent or independent whereas the termination in eukaryotes depends on polyA signal or terminator sequence.

2. False, Eukaryotes are highly evolved organisms which use three RNA pol for transcription compared prokaryotes which use only one.

3. False, the rho-dependent or rho-independent model of transcriptional termination occurs in prokaryotes but eukaryotes transcriptional termination depends on the polyA signal or terminator sequence.

4. True, Eukaryotic transcription initiation occurs through transcrition factors like TFIIA, TFIIB, TFIID, TFIIE and TFIIF, which form complex and bind to TATA box on the DNA sequence.

5. True, eukaryotic promoters have regulatory elements such as enhancers ans silencers.

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