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TRUE/FALSE and EXPLAIN. 1. You are provided with a number of tubes of melted Pla

ID: 58431 • Letter: T

Question

TRUE/FALSE and EXPLAIN.

1. You are provided with a number of tubes of melted Plate Count Agar (PCA) in a 50 degree celcius water bath for use in pouring plates.

Statement 1: Is Plate Count Agar an example of a differential medium?

Statement 2: Would we expect to obtain the same number of colonies on our plates if the tubes each contained twice as much medium?

2. Is colony-forming unit (CFU) microscopic, as is a cell?

3. Does colony-forming unit (CFU) replicate to form a colony?

4. Is colony-forming unit (CFU) a term we only use when quantitatin bacteria (such as determining CFUs per ml or gram of a sample)?

5. Does the 100X objective lens on our microscopes allow us to observe baterial flagella?

6. When using the 100X objective lens, must we actually touch the slide or cover slip while in use?

Explanation / Answer

3. No-colony forming units are just indication for the presence of virus in the bacterial culture. They cannot form colonies.

4. Yes, CFU is a term to use when quantitative bacterial analysis.

5. Yes, 100X objective lens can magnify the bacterial flagella.

6. It is necessary to use oil while observing the specimen using 100X lens. So, it is necessary to touch the slide or cover the slip while in use.