Which (if any) of the following statements regarding classicDNA experiments is n

Question

Which (if any) of the following statements regarding classicDNA experiments is not correct: A. phage protein contains sulfur B. treatment of cell extracts with enzymes that destroy DNAwill prevent transformation C. phage DNA labeled with radioactive phosphorus will entercells during infections D. treatment of cell extracts with protease will not preventtransformation E. all of the above are true Which (if any) of the following statements regarding classicDNA experiments is not correct: A. phage protein contains sulfur B. treatment of cell extracts with enzymes that destroy DNAwill prevent transformation C. phage DNA labeled with radioactive phosphorus will entercells during infections D. treatment of cell extracts with protease will not preventtransformation E. all of the above are true

Explanation / Answer

      In the given options allthe following statements regarding classic DNA experiments arecorrect.       Hence, the right option isE. all of the above aretrue.       This is because, option Athe phage protein contains sulfur is true.       In Hershy and Chase experiment,in head of phage molceule the proteins are radiolabeled withS34 and the phosphate        group of theDNA is labeled byP32.       Therefore option A thatphage protein contains sulfur is true.       Option B and D:       Avery, Mc Leod and McCarty in their experiments which they comtinued on the Griffiths,found that, when the        transformingmaterial was treated withproteases and RNases the transforming principle has beenobserved, whereas        when treated with DNases transformationhas not been observed.       But they statedexplicitily that DNA is the genetic material.       Hence, this indicates thattreating the cell extracts with enzymes that destroy DNA preventtransformation and treating       the cell extractswith enzymes called proteases will not preventtransformation       Therefore option B andD are true.       Option C:       In Hershy and Chaseexperiments, the DNA of the phage molecule has been labeled byP32 and the proteins were       labeled byS34.       Such radiolabeled phagemolecule has been cultured in a medium and in the progeny theradiolabeled P32 has been       observed.       As DNA is the geneticmaterial, the phage DNAthat is labeled with radioactive phosphorous will enter thecells during        infections.       Hence option C is alsotrue.       Therefore basing on allthese reasons option E is right.       Hope you are satsifiedwith the answer.

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