say the experiment was performed without the X-Gel indicator substrate in the ag

Question

say the experiment was performed without the X-Gel indicator substrate in the agar plates. all of the colonies from each ligation will be white as the blue colour cannot be seen in the absent of hydrolyzed X-Gel. if you were given the plates of white colonies from all three ligation reactions could you tell which was which and explain briefly each of the three transformation plate results? (clue: Numbers of transformants will be much larger for one type of ligation; moderate in numbers for the second type of ligation; and very low or absent for the third type of ligation.)

Explanation / Answer

Numbers of transformants will be much larger for one type of ligation and that type of ligation is lambda DNA . The number of transformants which is moderate in numbers for the second type of ligation is HindIII and the number of transformants which is very low or absent for the third type of ligation is pUC18.

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