35. A microbiologist transfers and inoculum from a slantlabeled Staphylococcus a

Question

35. A microbiologist transfers and inoculum from a slantlabeled Staphylococcus aureus on to a Petri plate. Followingincubation, both Staphylococcus aureus and Escherichia coli aredetermined to be growing in this culture. What is the most likelyexplanantion? a. the microbiologist used a complex media b. the culture is contaminated c. the incubation temperature was incorrect d. the culture medium must be selective e. the culture medium must be differential 40. In preparing a "Lab Report of a Bacterial Unknown" theLaboratory supervisor advised students that DNA testing would notbe available. Given this news, what other pure culturecharacteristics of an organism can be studied and evaluated: a. macroscopic b. microscopic c. biochemical d. all of the above e. none of the above 35. A microbiologist transfers and inoculum from a slantlabeled Staphylococcus aureus on to a Petri plate. Followingincubation, both Staphylococcus aureus and Escherichia coli aredetermined to be growing in this culture. What is the most likelyexplanantion? a. the microbiologist used a complex media b. the culture is contaminated c. the incubation temperature was incorrect d. the culture medium must be selective e. the culture medium must be differential 40. In preparing a "Lab Report of a Bacterial Unknown" theLaboratory supervisor advised students that DNA testing would notbe available. Given this news, what other pure culturecharacteristics of an organism can be studied and evaluated: a. macroscopic b. microscopic c. biochemical d. all of the above e. none of the above

Explanation / Answer

the first part is that, most likely, culture wascontaminated. Selective agars tend to show a preference forgrowing one type of bacteria over another. Differentialindicate differences (generall) and complex media is just that... alot of unknown stuff. Temperature would not be an inhibitoryfactor for either of these microorganisms. The second part is that all three can be used. Macroscopicobservation can show differences in culture types such as color,colony shape, or media preferences. Microscopic can showformation of cells, shape, and even motility (or lackthereof). Biochemical tests can indicate such functions asmannitol fermenation, utilization of catalase, or even staining ofthe cell wall. Hope this helps.

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