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I need help solving some generation time practicequestions. I have an exam on Mo

ID: 5800 • Letter: I

Question

I need help solving some generation time practicequestions. I have an exam on Monday and myprofessor is going to have some problems about this topic. Canyou help???
Example 1: If you have a generation time of20 mins, if you started with 5 cells, how many cells would youhave in 200 minutes? Example 2: A culture cotains 17 x 2^8 cells. How manycells did that culture contain six generations earlier? Example 3: You have a culture with 19 cells in it. Theculture goes through three generations. How many cells are now inthe culture (assume that the culture is in exponential phasethroughout the experiment, only viable cells are being measured andare of any concern to you, and that three generations implies threerounds of replication)? Example 4: Starting with 10 bacteria, a doubling time of30 minutes, and given exponential growth, how many bacteria willyou have after 3000 minutes (a little over 2 days) of continuousexponential growth? I need help solving some generation time practicequestions. I have an exam on Monday and myprofessor is going to have some problems about this topic. Canyou help???
Example 1: If you have a generation time of20 mins, if you started with 5 cells, how many cells would youhave in 200 minutes? Example 2: A culture cotains 17 x 2^8 cells. How manycells did that culture contain six generations earlier? Example 3: You have a culture with 19 cells in it. Theculture goes through three generations. How many cells are now inthe culture (assume that the culture is in exponential phasethroughout the experiment, only viable cells are being measured andare of any concern to you, and that three generations implies threerounds of replication)? Example 4: Starting with 10 bacteria, a doubling time of30 minutes, and given exponential growth, how many bacteria willyou have after 3000 minutes (a little over 2 days) of continuousexponential growth?

Explanation / Answer

1) (200min) / (20min / generation) = 10 generations 5cells x 210 = 5.12x103(210means that it doubles 10 times; 1 generation time is the time forall the cells to replicated => doubles the number of cells) 2) (17x28) / 26 = 68cells 3) 19x23= 152 4) 3000min / 30min/generation = 100generations 10x2100= 1.268x1031

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