1. Which of the following distinguishes the sequences and function of prokaryoti
ID: 57958 • Letter: 1
Question
1. Which of the following distinguishes the sequences and function of prokaryotic from eukaryotic promoters?
Only prokaryotic promoters contain conserved sequences
Only eukaryotic promoters contain conserved sequences
Only in eukaryotes are accessory proteins required for binding of RNA polymerase
Only in prokaryotes are accessory proteins required for binding of RNA polymerase
None of the above
Answers (1) and (4)
Answers (2) and (3)
2. Amongst RNAs, which of the following RNA types found in cells are relatively unstable?
mRNA
rRNA
tRNA
snRNA
All are equally unstable
Answers (2) and 3)
Answers (2), (3), and (4)
3. What is relationship between poly (A) tail length and mRNA translation in eukaryotes?
There is no relationship
Poly (A) tails are involved in binding of mRNA to the tRNAs
Long poly (A) tails impart shortened translation to mRNAs
Long poly (A) tails impart lengthened translation to mRNAs
There are no poly (A) tails in eukaryotic mRNAs, they are only in prokaryotic mRNAs
4. Small nuclear RNAs (snRNAs) play a role in which of the following processes?
DNA replication functioning as primers
RNA synthesis functioning as transcription initiation factors
RNA processing functioning as catalysts of intron removal
Translation by linking the mRNA with the ribosome
Addition of the 5’-7-methylguanine (CAP)
5. What is the role of sigma protein in mRNA synthesis in prokaryotes?
Forms the primer for mRNA synthesis
Termination of mRNA synthesis
Binding of RNA polymerase to promoters
Forming stem-loop structures in mRNA
Degradation of mRNA
Protection of mRNA from degradation
None of the above, sigma protein has no role in mRNA synthesis
Explanation / Answer
1. The correct option is None of the above
Both the prokaryotes and eukaryotes contain conserved sequences, and need accessory proteins for RNA polymerase binding.
2. The correct answer is mRNA
mRNA is the most unstable type of RNA in a cell. In bacteria, the mRNA has a half-life of only a few minutes.
3. The correct answer is There is no relationship
There is no relationship between poly (A) tail length and mRNA translation in eukaryotes. Poly A tail is added to the 3’end of the pre mRNA and protects the mRNA from enzymatic degradation, and also helps in the export of mature mRNA to the cytoplasm. Apart from this, the poly A tail length does nothing in mRNA translation.
4. The correct option is RNA processing functioning as catalysts of intron removal
A small nuclear RNA (snRNA) is one of many small RNA species that are found in nucleus; they are mainly involved in splicing or other RNA processing mechanisms.
5. Sigma protein allows the RNA polymerase to bind to the promoter. Once bound and initiates the transcription, the sigma protein is detached from the RNA polymerase.
Hence, the correct option is Binding of RNA polymerase to promoters.
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