Scan graph 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0.0 400 450 500 550 600 650 700 750 W

Question

Scan graph 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0.0 400 450 500 550 600 650 700 750 Wavelength (nm) a.) At what wavelength would a visible analytical analysis be best? b.) From the spectrum, what might be the color of the flower? A 5.00 x 106 M solution of standard Daphne papyraceae extract was used to prepare a Beer's Law plot. The following dilutions were made: Final Dilution Volume Volume standard extract 1.00 mL 1.50 mL 2.00 mL 2.50 mL 3.00 mL Solution # 2 10.00 mL 10.00 mL 10.00 mL 10.00 mL 10.00 mL 4 6 c.) Using this data, complete the following data table and prepare a suitable Beer's Law plot on appropriate graph paper. Show dilution calculations for solution 4. It will be assumed that the remaining solutions were calculated in the same wa y. For your plot, use graph paper or a completely prepared and labeled plot using a computer resource. Engineering grid is acceptable but not preferred Prepare the plot using as much of the paper area as possible. Include acid labels including units and an appropriate title. 58

Explanation / Answer

Answer A: A visible analytical analysis would be best between the wavelengths 550nm to 690nm as the formation of the peak begins around 570nm and the peak is formed around 660-670nm.

Answer B: Since the peak is formed at around 660nm-670nm, the colour of the flower is mostly red. (since the wavelength of red colour ranges from 750nm to 620nm)

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