1. In this experiment, you will need to create known standard concentrations in
ID: 574995 • Letter: 1
Question
1. In this experiment, you will need to create known standard concentrations in o rder to generate a standard Beer's law curve (Figure 2.2). You will need four different solutions of Fe2" that have concentrations in the range from 1.79 x 10-M to 1.25 x 10-*M. Assuming you want to make 10 mL. of each solution, calculate how many mL of a 1.79 x 10-* M F stock solution are necessary for each. (Note: Graduated cylinders will be used to these volumes, so think about the level of precision possible in choosing volume amounts) dispenseExplanation / Answer
Solution:- This is dilution problem so we can can use the equation, M1V1 = M2V2
We want to make 10 mL of 1.79 x 10-5 M and 1.25 x 10-4 M solutions from 1.79 x 10-4 M solution.
Calculations for the first one would be as...
1.79 x 10-4 M x V1 = 1.79 x 10-5 M x 10 mL
V1 = 1.79 x 10-5 M x 10 mL/ 1.79 x 10-4 M
V1 = 1 mL
So, for making 10 mL of 1.79 x 10-5 M solution we need 1 mL of 1.79 x 10-4 M solution.
calculations for the second solution would be as....
1.79 x 10-4 M x V1 = 1.25 x 10-4 M x 10 mL
V1 = 1.25 x 10-4 M x 10 mL/ 1.79 x 10-4 M
V1 = 6.98 = 7 mL
so, for making 10 mL of 1.25 x 10-4 M we need almost 7 mL of 1.79 x 10-4 M solution.
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