This pre-lecture quiz will cover material related to the syllabus as well as mat

Question

This pre-lecture quiz will cover material related to the syllabus as well as material from the upcoming lab (FRET Sensor GE Purification) Your email address (fishe801@d.umn.edu) will be recorded when you submit this form. Not you? Switch account * Required Question 4. An optical density at 600nm is used during E. point coli culturing for what purpose? O Measuring metabolism of E. Coli as an indicator of cell proliferation O Light scattering from E. coli for cell density determination O The fluorescence of the GE FRET probe to measure protein content O Because the Boersma lab published using this wavelength in their method Question 4. What is the largest, SINGLE contributor to your 1point

Explanation / Answer

For finding bacterial cell density the phenomenon of light scattering is made use of. E.Coli. is also a bacterium.

And about choosing the wavelenth of 600nm, the logic is that the bacteriological media are yellow in colour and therefore will have low absorbance and high scatterring at around 600nm.

That's why OD 600 is chosen during E.Coli. culturing for the purpose of light scattering from E.Coli for cell density determination.

Related Questions

Navigate

• Browse (All)
• Browse T
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.