Ribonuclease, an enzyme that catalyzes the hydrolysis of RNA, has a molecular we

Question

Ribonuclease, an enzyme that catalyzes the hydrolysis of RNA, has a molecular weight of 13,800 and an E^1% 280 of 7.3. How many tyrosine so does it contain? What assumptions must you make to solve this problem? Why do you think that the answer is not an exact integer? Ribonuclease, an enzyme that catalyzes the hydrolysis of RNA, has a molecular weight of 13,800 and an E^1% 280 of 7.3. How many tyrosine so does it contain? What assumptions must you make to solve this problem? Why do you think that the answer is not an exact integer?

Explanation / Answer

The formula for calculation is:

Molar Extinction Coefficient of Tyrosine of a protein () = (Number of Tryptophan residues × 5500) + (Number of Tyrosine residues × 1490)

Thus,

molar of Tyrosine = (1 × 5500) + (5 × 1490) = 12950

The amino acid sequence of Ribonuclease is   

It has,

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