a) What is the generation time (g) of this culture? c) A sample of this culture

Question

a) What is the generation time (g) of this culture?

c) A sample of this culture taken at 10h is diluted by a factor of 106X, after which 0.1 ml are spread on a plate. How many CFUs (colony-forming units) are predicted?

d) A 0.1ml sample taken at 1h is applied to the counting chamber of a hemacytometer. What would be the average number of cells expected in a square with the following dimensions: 1.0mm X 0.2mm X 0.01mm?

e) A sample taken at 6h had an optical density 0.4. What would be the predicted optical density at 8h?

Explanation / Answer

To determine generation time we have to determine growth rate k

K= log10 [xt] - log 10 [xo]÷ 0.301 × t

Generation time = 1/k

Here 1.00E+ 0.6 = 106

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