(16 pts) 50 mL of tissue culture medium buffiered hy the bicarhonate CO buffier
ID: 569983 • Letter: #
Question
(16 pts) 50 mL of tissue culture medium buffiered hy the bicarhonate CO buffier system is used to grow liver hepatocytes. Cells are grown in this medium inside vontad tels man atmosphere of 92% air and 8% co," Under this condition at 37 the total arbonat pool is 26,51 mmalesliter. The graphs labeled A-D represent the response of this buffer under conditions that affest gas esschange. 8.00 7.50 7.00 PH 6.50 6.00 5.50 5.00 0 0.005 01 oi5 002 0.025 moles H+ produced Based on the data, which system, A, B CrDis least suited for cultivation of these cells? Answer: Which system appears best for cultivation of these celis Answer: Compared to graph A, what is the best reason for the oerved response to added acid in curves B, and D? A. Graphs B, C, and D represent systems with decreasing gas exchange. B. Graphs B, C, and D represent systems with increasing gas exchange. C. Graphs B, C, and D represent systems with inibial diffèrent composition. D. Cannot determine any difference based on the da Compared to graph A, the response of graphs B,C, and Dto skled H+ indicates that these systems behave as though A the pKa of the buffer is increased compared to gm A. B, the pKa of the buffer is decreased compared to graph A C the pKa is equivalent to the pKa in graph A.Explanation / Answer
1. Cultivation of liver hepatocytes
a) based on the graph, the least suited system for the cultivation would be,
A
b) System best for cultivation of cells
D
c) Compared to A, the best reason for oberseved graphs B, C and D are,
A.
d) Compared to A, graphs B, C and D to H+ indicate the behaviour as though,
C.
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