2) A TLC is spotted with 1 lane being an unknown sample and another lane being a

Question

2) A TLC is spotted with 1 lane being an unknown sample and another lane being a reference compound you suspect the unknown to be. You recall that the position of the spots on the TLC plate are specific to each compound and that all components of any spotted solution show up on a TLC. After running the TLC, you find that your sample lane has multiple spots which situate near the very top and bottom of the plate. You also find that the reference standard situates near the middle. Given this information, did you actually have the compound you suspected and did you get a pure sample? 3) After performing the entire experiment, you find that 12% of the mass of your tea leaf sample was actually caffeine. If you starting with 5g of tea leaves at the beginning, what is the mass of caffeine obtained?

Explanation / Answer

2) Since there was no spot of sample at similar level to that of reference, the suspected molocule was no present in the sample.

3) 12% of 5g = 0.6g

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