experiment effect of enzyme on the rate 1. write a properly worded hypothesis re

Question

experiment effect of enzyme on the rate
1. write a properly worded hypothesis regarding the effect of enzyme concentration on the rate of reaction ( experiment effect of enzyme on the rate of reaction )
2. based upon your hypothesis write a properly worded prediction concerning the expected outcome of the experiment.
3. explain the role of guaiacol in this experiment ( think not only about the chemical reaction. but also about the products that are formed and how you are physically measuring the rate of the reaction.
4 why was it important to keep the test tube containing the enzyme extract and the substrates separate until we are ready to take the absorbance readings?
5 . explain the purpose of test tube that contained buffer but no enzyme extract. what was it used for ( don't juts tell me it was a control?

Explanation / Answer

1. Testing Hypothesis - The rate of the reaction varies proportionately to the concentration of the enzyme

2. If the hypothesis is true, then we will see a change in absorbance at 470nm proprotional to the change in concentration of the enzyme.

3. The reaction generates oxygen. Guaiacol  is used as an indicator. When oxygen binds to it, the complex turns yellowish brown and absorbs light maximally at about 470 nm. This is how we physically measure the rate of the reaction.

4. The rate of the reaction is very fast, and if we mix the two, the readings are to be taken instantaneously, else the reaction will reach steady state equilibrium, and the rate will not be known.

5. It is there to check whether the guaiacol is okk or not, i.e. what is the minimal absorbance value that is to be substracted from all the experimental values to get the true rate. AND ... it is aso a negative control :)

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