3. Use the ncbi website and other internet sources to identify the following seg

Question

3. Use the ncbi website and other internet sources to identify the following segment of a eukaryotic gene’s protein sequence (20 amino acids).
http://www.ncbi.nlm.nih.gov/

Amino end- DINVYGDVVINHKGGADATE   -carboxy end

A) Write an mRNA sequence that could code for this segment of protein (Note: these are single letter amino acid symbols for 20 amino acids)

B) Write the double stranded DNA sequence of the gene region that codes for this segment of protein.

C) Write the tRNA anticodon sequence that corresponds to the mRNA sequence coding for this segment of protein.

Explanation / Answer

This sequence could be found on motor domain of kinesin protein (ncbi website).

A) Asp- Ile-Asn- Val-tyr-gly-asp-val-val-iso-asn-his-lys-gly-gly-Ala-Asp- Ala- Thr-Glu

5` end---GAUAUCAAUGUUUAUGGAGAUGUUGUUAUCAAUCAUAAAGGAGGAGCGGAUGCGACUCAA-- 3`end

B) 5` end---GATATCAATGTTTATGGAGATGTTGTTATCAATCATAAAGGAGGAGCGGATGCGACTCAA-- 3`end

     3` end---CTATAGTTACAAATACCTCTACAACAATAGTTAGTATTTCCTCCTCGCCTACGCTGAGTT--5`end

C) tRNA anticodon is opposite to the mRNA codon sequence.

CUAUAGUUACAAAUACCUCUACAACAAUAGUUAGUAUUUCCUCCUCGCCUACGCUGAGUU-

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