e protein bovine serum albumin (BSA) has been prepared at u have a supply of thi
ID: 566771 • Letter: E
Question
e protein bovine serum albumin (BSA) has been prepared at u have a supply of this A stock solution of th a concentration of 20 milligrams per milliliter (mg/ml); yo stock provided for you at your station. Bring a 30 ml supply of distilled water to your desk. Use the serial dilution technique to prepare 1.5 ml volumes of the various BSA concentrations listed below. Write in the space provided, how you will prepare each of the sample preparations. B concentration with solution from a different concentration. e careful not to contaminate oneExplanation / Answer
To prepare a set of solution from stock
stock BSA solution = 20 mg/ml
Desired concentration = 10 mg/ml
Final volume = 1.5 ml
So,
volume of 20 mg/ml stock added = 10 mg/ml x 1.5 ml/20 mg/ml = 0.75 ml
Take 0.75 ml of 20 mg/ml stock and add 0.75 ml water to it.
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Desired concentration = 5 mg/ml
Final volume = 1.5 ml
So,
volume of 20 mg/ml stock added = 5 mg/ml x 1.5 ml/20 mg/ml = 0.375 ml
Take 0.375 ml of 20 mg/ml stock and add 1.125 ml water to it.
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Desired concentration = 2.5 mg/ml
Final volume = 1.5 ml
So,
volume of 20 mg/ml stock added = 2.5 mg/ml x 1.5 ml/20 mg/ml = 0.1875 ml
Take 0.1875 ml of 20 mg/ml stock and add 1.3125 ml water to it.
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Desired concentration = 1.25 mg/ml
Final volume = 1.5 ml
So,
volume of 20 mg/ml stock added = 1.25 mg/ml x 1.5 ml/20 mg/ml = 0.094 ml
Take 0.094 ml of 20 mg/ml stock and add 1.406 ml water to it.
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