1) To measure the activity ofperoxiase enzyme from a turnip, you must mix citrat

Question

1) To measure the activity ofperoxiase enzyme from a turnip, you must mix citrate pH buffer, guaiacol substrate (clear; turns brown) hydrogen peroxide substrate (clear) and turnip extract (pinkish-tan). Which of the following would you include in the BEST NEGATIVE control as a BLANK for spectrophotometry?

a) A) Citrate buffer

b) B) Peroxidase

c) C) Guaiacol

d) D) Turnip extract

e) All of the above

f) F) A,B, and C

g) G) A, C, and D

2) You seperate mitochondrial proteins by polyacrylamide gel electrophoresis. You immediately notice Cytochrome C in the gel, made visible by the red cytochrome electron carriers that it contains as coenzymes. Upon completion of the electrophoresis, the bromophenol blue dye front has traveled 95mm towards the anode, but Cytochrome C has traveled only 19mm. Cytochrome C is a relatively _______________________ protein.

a) Dense

b) Large

c) Small

d) Acidic

e) Anionic

f) Unstable

g) Low molecular weight

Explanation / Answer

1. A, C,and D

The solvent used in the experiment can be as blank to serve as a reference tube.

2. Anionic

Cytochrome c is negatively charged should moves towards cathode

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