Please complete the table below and show your work on a separate sheet of paper.

Question

Please complete the table below and show your work on a separate sheet of paper. Note that the stock solution of BSA is 2.0 mg/ml, Final (uL) 100 ul. Tube Volume Volume of Final BSA Amount of 2.0mg/mL BSA Volume Concentration BSA in 100 (ug/mL) stock (HL) dH2O 100 ul of stock | 2.000 ug/mL 200 Hg of stock | 100 . | 1.500 ug/mL | 1.000 ug/mL | 750 ug/mL | 500 ug/mL 250 ug/mL | 125ug/mL | 25 ug/mL | 100 ul. ul. of stock | 100 ul. of stock | 100 ul. ul. of stock of stock | 100 .of stock | 100. of stock | 100 ul.

Explanation / Answer

Given,

concentration of stock BSA solution = 2.0 mg/ml = 2,000 ug/ml

1 ml = 1000 uL

So,

Tube B

Volume of BSA stock = 1,500 ug/ml x 100 uL/2,000 ug/ml = 75 uL

BSA in 100 uL = 150 ug

Tube C

Volume of BSA stock = 1,000 ug/ml x 100 uL/2,000 ug/ml = 50 uL

BSA in 100 uL = 100 ug

Tube D

Volume of BSA stock = 750 ug/ml x 100 uL/2,000 ug/ml = 37.5 uL

BSA in 100 uL = 75 ug

Tube E

Volume of BSA stock = 500 ug/ml x 100 uL/2,000 ug/ml = 25 uL

BSA in 100 uL = 50 ug

Tube F

Volume of BSA stock = 250 ug/ml x 100 uL/2,000 ug/ml = 12.5 uL

BSA in 100 uL = 25 ug

Tube G

Volume of BSA stock = 125 ug/ml x 100 uL/2,000 ug/ml = 6.25 uL

BSA in 100 uL = 12.5 ug

Tube H

Volume of BSA stock = 25 ug/ml x 100 uL/2,000 ug/ml = 1.25 uL

BSA in 100 uL = 2.5 ug

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