help What are the two dyes used for staining in this experiment and what so they
ID: 56037 • Letter: H
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help What are the two dyes used for staining in this experiment and what so they stain? What is function of SDS and 0.5M NaCI in the isolation of DNA How did DNase affect the spooling of DNA? What % of ethanol was used in the DNA isolation and where did stand when added to aqueous solution and why? How did the chromatin in the nucleus appear after the addition of SDS, DNAse and 0.5M NaCl What are the different methods used for breaking the cells open? What is the relation between RPM and RCF? What RCF is used for the separation of nuclei, mitochondria, microsomes and ribosomes? Calculate the RCF if the revolution per minute of the rotor is 1000 and the radius is 100mm. Calculate the RPM if the radius of the rotor is 75mm and the RCF is 550 xG What type of centrifuge, rotor and tubes did you use for nuclei separation? What necessary step do you need to take before keeping the centrifuge tubes in the rotor and starting the centrifugation?Explanation / Answer
8. The outer cell wall is madeup of a phospholipid layer, which has to be broken to expose the cellular organelles. Several methods are generally employed to lyse the cell membranes, some of them are given below.
A. Mechanical breaking (No chemicals are used)
B. Breaking by mortar and pestle
C. Breaking using glass or ceramic beads.
D. By using ultrasonic homogenisers.
E. Freezing, and homogenization
F. Enzymes such as chitinase, lysozyme, mannase etc.
G. Using alcohols and aromatic solvents such as ether and chloroform.
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