1. The wildtype sequence of partof a protein is: NH 2 -Trp-Trp-Trp-Met-Arg-Glu-T

Question

1.      The wildtype sequence of partof a protein is:

NH2-Trp-Trp-Trp-Met-Arg-Glu-Trp-Thr-Met…

Each mutant in the following table differs from wildtypeby a single point mutation (base substitution). Using thisinformation, determine the mRNA sequence coding for the wildtypepolypeptide. If there is more than one possible nucleotide,list all possibilities.

Mutant

Amino acid sequence of polypeptide

1

Trp-Trp-Trp-Met

2

Trp-Trp-Trp-Met-Arg-Asp-Trp-Thr-Met

3

Trp-Trp-Trp-Met-Arg-Lys-Trp-Thr-Met

4

Trp-Trp-Trp-Met-Arg-Glu-Trp-Met-Met

Mutant

Amino acid sequence of polypeptide

1

Trp-Trp-Trp-Met

2

Trp-Trp-Trp-Met-Arg-Asp-Trp-Thr-Met

3

Trp-Trp-Trp-Met-Arg-Lys-Trp-Thr-Met

4

Trp-Trp-Trp-Met-Arg-Glu-Trp-Met-Met

Explanation / Answer

ANSWER :

NH2-Trp-Trp-Trp-Met-Arg-Glu-Trp-Thr-Met…

Trp—UGG

Met- AUG

Arg/R CGU, CGC, CGA, CGG, AGA, AGG

Asp/D GAU, GAC

START --AUG

STOP UAA, UGA, UAG

Glu GAA, GAG

LYSINE -AAA,AAG

Thr/T ACU, ACC, ACA,ACG
=====================================

Trp-Trp-Trp-Met

UGG UGG UGG AUGCGA

UGG UGG UGGAUG UGA

NH2-Trp-Trp-Trp-Met-Arg-Glu-Trp-Thr-Met…

UGG UGG UGG AUG CGAGAA, UGG AUG

UGG UGG UGG AUG CGAGAU, UGG AUG

Trp-Trp-Trp-Met-Arg-Asp-Trp-Thr-Met

NH2-Trp-Trp-Trp-Met-Arg-Glu-Trp-Thr-Met…

UGG UGG UGG AUG CGA GAA, UGG AUG

UGG UGG UGG AUG CGA AAA, UGG AUG

Trp-Trp-Trp-Met-Arg-Lys-Trp-Thr-Met

NH2-Trp-Trp-Trp-Met-Arg-Glu-Trp-Thr-Met…

UGG UGG UGG AUG CGA GAA, ACG AUG

UGG UGG UGG AUG CGA GAA, AUG AUG

Trp-Trp-Trp-Met-Arg-Glu-Trp-Met-Met

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