cell biology Your friend sends you two tubes of protein. One tube contains a pro
ID: 55749 • Letter: C
Question
cell biology Your friend sends you two tubes of protein. One tube contains a protein, Crg1, that contains a nuclear export signal. The other tube contains the nuclear export receptor. Xpo1, for Crg1. You are excited to have received these proteins, because you are interested in the biochemistry of nuclear export. You try to get Crg1 to bind Xpo1 but do not succeed. You complain to your lab partner about how your friend sent you bad reagents. However, your lab partner has just taken a cell biology class, and gently reminds you to try one more experiment before you call your friend. What does your labmate suggest?Explanation / Answer
In in vivo, the binding of Xpo1 to Crg1 requires Ran-GTP. In the experiment, you have only added Xpo1 to Crg1, so due to lack of Ran-GTP the Xpo1 and Crg1 would not bind each other. So, you lab mate may suggest that addition of Ran-GTP to the reaction sample facilitate the binding of Xpo1 and Crg1.
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