2. If the following mix of molecules were purified using size exclusion chromato

Question

2. If the following mix of molecules were purified using size exclusion chromatography, what would be the order in which the molecules are eluted (which comes out first, second, and last)? Mixture: hemoglobin, 65,000 daltons; myoglobin 17,000 daltons; myosin, 180,000 daltons. 3. Consider the following analysis. An unknown protein has a retention time of 6.3 minutes when analyzed by size exclusion HPLC. Using your graph what is the molecular weight of the protein? 4. An unknown protein was found to have a molecular weight of 170,000 daltons based on size exclusion HPLC. When the same protein was analyzed by SDS- PAGE two bands were visible. Comparison to standard proteins revealed one band had a molecular weight of 100,000 and the other band had a molecular weight of 35,000. What is the subunit composition of the protein? 119

Explanation / Answer

Myosin will elute first, after that hemoglobin and myoglobin at last.

Explanation:

Size Exclusion Chromatography (SEC) is a chromatographic method in which molecules are separated on the basis of their exclusion from pores in the column packing material. Larger analytes will elute first, while the smaller molecules interact more with the stationary phase and will elute later.

Only one question will be answered at a time as per Chegg policy.

Related Questions

Navigate

• Browse (All)
• Browse 2
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.