Map Shown below are chromatograms for the separation of compounds A and B obtain

Question

Map Shown below are chromatograms for the separation of compounds A and B obtained at the same flow rate from two different columns of equal length. The void time, m, is equal to 1.75 min on each column nm Column 1 Column 2 5 15 20 25 Time (min)- What is the value of the adjusted relative retention for the two compounds separated using column 2? Number Scroll down to view the enitre problem. = 1 1.33 Which compound has a larger retention factor? O They are the same O Compound A O Compound B a smaller partition coefficient? O Compound A O They are the same O Compound bB Previous Give Up & View Solution e Check Answer ) Next Exit Hint

Explanation / Answer

The value of the adjusted relative retention for two compounds using column 2 maybe nearly 1. 33 min.

The compound B has larger retention time because this component elated last, that is this compound has more adsorption than A so retained.

Compound A has smaller partition coefficient hence elated firstly. Partition coefficient = concentration of A in stationary phase /concentration of A in mobile phase.

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