Explain in detail each answer in the Chart Please and Thank you. Characteristic
ID: 55543 • Letter: E
Question
Explain in detail each answer in the Chart Please and Thank you.
Characteristic
Genomic Synthesis
Antigenomic Synthesis
mRNA transcription
1
RNA polarity (+ or -)
Positive
Negative
Positive
2
Sensitivity to a-amanitin (yes or no)
Yes
Yes
NO
3
Small delta antigen requirement (yes or no)
Yes
NO
YES
4
Polymerase
RNAP II
RNAP I
RNAP II
5
Site of synthesis
Nucleoplasm
Nucleolus
Nucleoplasm
Characteristic
Genomic Synthesis
Antigenomic Synthesis
mRNA transcription
1
RNA polarity (+ or -)
Positive
Negative
Positive
2
Sensitivity to a-amanitin (yes or no)
Yes
Yes
NO
3
Small delta antigen requirement (yes or no)
Yes
NO
YES
4
Polymerase
RNAP II
RNAP I
RNAP II
5
Site of synthesis
Nucleoplasm
Nucleolus
Nucleoplasm
Explanation / Answer
It is the condition utilized for HDV transfection of mammalian cells. The RNA polarity is positive for cDNA mediated transfection and negative for RNA mediated transfection. cDNA mediated transfection is sensitive to a-amanitin and it requires small delta antigen and the polymerase is RNAP II and the site of synthesis is nucleoplasm, where as in RNA mediated transfection it does not require small delta antigen and it needs RNAP I and the site of synthesis is nucleolus.
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